Investigation of the Statistical and Spatial Distributions of Mercury Contaminated Fish, Surface Waters and Soils in Arkansas

Mercury (Hg) contamination of fish is a widespread problem throughout much of the United States and the world (Louisiana WWW page, 1997). Levels ofHg in fish suffic1ent to exceed the FDA action level of 1 mg kg-1 have been found in many water bodies, including some in Arkansas and Louisiana. As a result of the serious public health ramifications for developing fetuses and for people that subsist on native fish, fish consumption advisories due to Hg contamination have been issued in 29 states. Contamination of surface water bodies by Hg results from deforestation, forest fires, fossil fuels, mining, natural emissions and commercial emissions (Armstrong, 1994). In addition, Hg has a high affinity for organic matter in soil and sediments, and therefore, long-term storage of Hg is an environmental problem. An excellent review of the integration and synthesis of recent work on Hg pollution is given in several papers edited by Watras and Huckabee (1994). The general consensus of the reports in this document seems to be that increases in Hg levels can be attributed to one or more of several mechanisms including atmospheric deposition, acidification of soils and lakes by sulfur deposition followed by an increased sulfate reduction, and transport from other source areas

GIS Characterization of Beaver Watershed

Beaver Reservoir watershed is located in Northwest Arkansas including portions of Madison, Washington, Benton, Carroll, Franklin and Crawford counties. This watershed is important to the Northwest Arkansas region because it supplies most of the drinking water for the major towns and cities, and several rural water systems. The watershed consists of 308,971 ha with elevations ranging from approximately 341 m to 731 m above mean sea level. It includes the Springfield Plateau and the Boston Mountains provinces within the Ozark Plateau physiographic region. There are approximately 581 km of streams, 532 km of shore line, and 3712 km of roads in the watershed most of which are city streets and rural roads. The soils in the watershed vary extensively and are quite complex due to the differences in parent material, topography and time. Most parent material of the soils in the Springfield Plateau is limestone, whereas in the Boston Mountains the dominant parent material is sandstone and shale. The differences in soils have led to the differences in landuse and land cover. The near surface geology in the watershed is also divided by physiographic provinces. Most of the Springfield Plateau surface geology is limestone, whereas the Boston Mountains are primarily sandstone and shale. Spatial details of the streams, roads, soils and geology attributes in the watershed are presented in this report. The GIS database and characterization of the watershed offers an excellent beginning to future research and modeling of various water quality parameters in this and other watersheds

Identification of Optimal Locations for Sampling Ground Water for Pesticides in the Mississippi Delta Region of Eastern Arkansas

Concerns about the presence of pesticides in the Mississippi River Valley alluvial aquifer in the Arkansas Delta have generated the need to develop a map of ground water vulnerability for this region comprised of approximately 10 million acres. Based on the availability of digital data and the scale of this study. we used a modified Pesticide DRASTIC model in a GRASS GIS environment to identify areas that were physically more sensitive to pesticide contamination than other areas within the Delta. Spatial distribution of pesticide loading was estimated from pesticide application rates in different crops and crop distribution map interpreted from satellite imagery. Relative ground water vulnerability index was expressed as a product of aquifer sensitivity index and pesticide loading index. The resulting map showing the spatial distribution of relative ground water vulnerability index values was intended for use in selecting optimal locations for sampling ground water for pesticides in the Arkansas Delta and for aid in implementing the Arkansas Agricultural Chemical Ground-Water Management Plan. The most sensitive areas in the Delta are distributed mostly along major streams where a combination of shallow depth to ground water, thin confining unit, permeable soils, and high recharge rate usually prevails. It is also in many of these areas where large acres of crops are grown, and pesticides are used. Consequently, many areas along major streams are also most vulnerable. These vulnerable areas may be targeted by planners and governmental agencies for further detailed evaluation. Uncertainties in the methodology and mapped input data, plus the dynamic nature of model factors, require continued and improved efforts in ground water vulnerability assessment for the Arkansas Delta

Prediction and Management of Sediments Load and Phosporus in the Beaver Reservoir Watershed Using A Geographic Information System

A study was conducted to compile a GIS database for the Beaver Reservoir Watershed and then use the database to run the Universal Soil Loss Equation and the Phosphorus Index Model on the War Eagle Creek Watershed, a portion of the Beaver Reservoir Watershed database. Characterization of the spat i a 1 properties of the primary attributes compiled for the watershed were reported. In addition, water quality samples taken from War Eagle Creek were analyzed for relationships across the watershed. Erosion in the watershed was lower than expected with well vegetated and fertilized pastures contributing to the reduction of annual sediment yield. The Phosphorus Index Model results showed that pastures in the watershed become highly vulnerable to phosphorus transport with small amounts of phosphorus, but only a small fraction of the watershed was classified as excessively vulnerable to phosphorus transport with excessive fertilizer application rates. Aqueous total phosphorus concentrations within the watershed showed normal seasonal variability with the exception of high concentrations at one site. Aqueous orthophosphorus concentrations also showed normal seasonal variations but few other conclusions could be drawn due to the overall low concentrations. There were few conclusive trends between phosphorus concentrations and attributes from the watershed database suggesting multiple sources contributed to the phosphorus in the War Eagle Creek

Spatial and Temporal Changes in Land Use and Land Cover from 1988 to 1992 in the Upper White River Watershed

Changes in Land Use and Land Cover (LULC) in the upper White River Watershed from 1988 to 1992 were analyzed and plotted using the geographic information system known as Geographic Resources Analyses Support System (GRASS). This portion of the White River Watershed includes two sub-basins the East Fork and the Middle Fork. In addition, LULC changes were determined for two smaller sub-basins, Shumate Creek and Cannon Creek, located in the East Fork of the White River. The sources and methods of interpretation of the 1988 and 1992 of data were different. Thus, there were variations in how certain portions of the vegetative cover were classified. Methods of classifying each of these are discussed in the following section

Soils of Mississippi County, Arkansas

Along with air and water, soil contributes essential processes to the natural order of global cycles. With the exception of edibles from the sea, virtually everything we, and most other land-based animals, eat is derived from soil. Soil is a storage medium of essential minerals and nutrients for fulfilling our agricultural and nutritional needs. Humans work the soil to provide the basics of food, clothing, and shelter. We also use the soil as a medium to store and discard our waste. Virtually everything we do is in some way connected to soi

Functional interaction between mouse erbB3 and wild-type rat c-neu in transgenic mouse mammary tumor cells

INTRODUCTION: Co-expression of several receptor tyrosine kinases (RTKs), including erbB2 and erbB3, is frequently identified in breast cancers. A member of the RTK family, the kinase-deficient erbB3 can activate downstream signaling via heterodimer formation with erbB2. We studied the expression of RTK receptors in mammary tumors from the wild-type (wt) rat c-neu transgenic model. We hypothesized that physical and functional interactions between the wt rat neu/ErbB2 transgene and mouse ErbB3-encoded proteins could occur, activating downstream signaling and promoting mammary oncogenesis. METHODS: Immunohistochemical and Western blot analyses were performed to study the expression of rat c-neu/ErbB2 and mouse erbB3 in mammary tumors and tumor-derived cell lines from the wt rat c-neu transgenic mice. Co-immunoprecipitation methods were employed to quantitate heterodimerization between the transgene-encoded protein erbB2 and the endogenous mouse erbB3. Tumor cell growth in response to growth factors, such as Heregulin (HRG), epidermal growth factor (EGF), or insulin-like growth factor-1 (IGF-1), was also studied. Post-HRG stimulation, activation of the RTK downstream signaling was determined by Western blot analyses using antibodies against phosphorylated Akt and mitogen-activated protein kinase (MAPK), respectively. Specific inhibitors were then used with cell proliferation assays to study the phosphoinositide-3 kinase (PI-3K)/Akt and MAPK kinase (MEK)/MAPK pathways as possible mechanisms of HRG-induced tumor cell proliferation. RESULTS: Mammary tumors and tumor-derived cell lines frequently exhibited elevated co-expression of erbB2 and erbB3. The transgene-encoded protein erbB2 formed a stable heterodimer complex with endogenous mouse erbB3. HRG stimulation promoted physical and functional erbB2/erbB3 interactions and tumor cell growth, whereas no response to EGF or IGF-1 was observed. HRG treatment activated both the Akt and MAPK pathways in a dose- and time-dependent manner. Both the PI-3K inhibitor LY 294002 and MEK inhibitor PD 98059 significantly decreased the stimulatory effect of HRG on tumor cell proliferation. CONCLUSION: The co-expression of wt rat neu/ErbB2 transgene and mouse ErbB3, with physical and functional interactions between these two species of RTK receptors, was demonstrated. These data strongly suggest a role for erbB3 in c-neu (ErbB2)-associated mammary tumorigenesis, as has been reported in human breast cancers